 | 01 September 2009 By:Kevin Altria
Describing the theoretical background on how the separations occur, the operating approaches that can be taken and method development.
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01 December 2008 By:Oscar Salas-Salano, Lynn Gennaro, Chantal Felten
Electrophoretic techniques, such as sodium dodecyl sulphate polyacrylamide (SDS-PAGE) and isoelectric focusing (IEF) gels, have traditionally been part of release testing in the biopharmaceutical industry. However, these slab gel methods are inconvenient and irreproducible because of the staining/destaining steps used in analyte detection, the use of toxic reagents and high intra- and inter-gel effective mobility variability.
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01 September 2008
Capillary electrophoresis (CE) is increasingly being used in vaccine analysis. Applications include vaccine quantification, purity assessment, process monitoring, characterization and nucleic acid analysis. CE has been used because it offers advantages over other techniques. Vaccine manufacturers such as Sanofi-Aventis, Merck and Wyeth are actively investigating the use of CE for vaccine analysis. While CE can be considered a mature technique, its application to vaccine release and characterization has been limited. It is difficult to ascertain if its use is expanding because there are still a small amount of publications, but given the inherent advantages of CE and the need for better analytical techniques in vaccine analysis, the application must be growing. This article illustrates some of the uses of CE in vaccine analysis.
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01 April 2008 By:Kevin Altria
Sample diluent ionic strength, pH, organic solvent composition and viscosity can all have a significant impact on the performance of CE separation. It is, therefore, essential that the choice of solvent is optimized during method development. The optimized diluent should be fully specified in the method and used consistently in method validation and all subsequent applications of the method.
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01 April 2007 By:Kevin Altria
Accurate preparation and correct selection of buffers is essential to obtain reproducible and consistent results in capillary electrophoresis (CE). A number of factors should be considered in buffer optimization, including the pKa of the buffering ion and the analyte as well as the molarity of the acid or base used in the adjusting procedure. Accurate recording of the precise reagents used and the procedures performed is necessary to prepare buffers consistently. "Poor reproducibility of results and poor quantitative precision will be attainable in CE assays without significant attention being paid to the preparation of buffers used."
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01 May 2005
The authors describe how this free downloadable software calculates and displays CZE separations after the user has entered sample and operations parameters. The underlying principles and mathematical basis are explained.
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01 January 2004

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01 March 2003
Guest author, Paul Cancalon, describes the role of capillary electrophoresis for the analysis of vitamins in citrus fruit juices. The separation is rapid with up to 30 citrus juice compounds capable of being separated in 20 min.
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01 January 2003
The authors provide an introductory description of MEEKC and highlight some of its applications. They demonstrate the separation possibilities offered by the technique for the analysis of pharmaceutical and non-pharmaceutical solutes.
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