Column: Coupling Matters - Chromatography Online

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Column: Coupling Matters
  • Molecular Interaction Sensors: A New Type of Detector for Separation Methods



    Potentiometry is a new detection method for liquid chromatography (LC) and capillary electrophoresis (CE). The principle behind this method is familiar to chromatographers because the signals depend on the partitioning tendency of analytes over the sensor coating and the eluent. This partitioning provokes a change in the surface potential and the detection of these changes can be classified as "potentiometric". A conversion algorithm is needed to convert the generated signals to concentration-related tracings (chromatograms).

    Gas Chromatographic Techniques for the Analysis of Chemical Warfare Agents



    The complexity of the systems that are used vary from standard GC configurations to assess the effectiveness of protective clothing to trace analysis of biomedical samples with complex GC configuration.

    Characterization of Triacylglycerides from Edible Oils and Fats using Single and Multidimensional Techniques


    Non-Discriminating Analytical Pyrolysis — A Novel Tool for Studying Environmental Samples


    Conventional pyrolysis can cause defunctionalization of polar structural moieties carrying functional groups, which leads to biased results.

    High-Throughput Peptide Mass Mapping using an Integrated Capillary Device Coupled to a Mass Spectrometer



    In this article, the author describes the development of a simple enzymatic reactor, which also functions as a microdevice that combines both preconcentration and enzymatic digestion in the nano-electrospray emitter, and its interfacing with a mass spectrometer.

    Multidimensional and Hyphenated Techniques in Aerosol Analysis



    The authors provide suggestions for the sample pretreatment and analysis of aerosol particles including the use of on-line SFE–LC–GC–MS, GCXGC and LC–MS. Although each technique has its merits, the novel multidimensional systems and those integrating sample pretreatment with the final analysis proved to be valuable tools in aerosol analysis.

    A Combination of Liquid Chromatography and Mass Spectrometry for Peptidomic Research



    The guest authors describe the use of LC–MS for the analysis of peptides. They deal with peptide extraction, peptidomic analysis in organisms with and without genomic databases, identification of peptides with conserved N- and C- terminal sequences, and in situ MALDI-TOF peptide profiling.

    Nomenclature and Conventions in Comprehensive Multidimensional Chromatography



    The authors describe recent discussions on the correct use of terminology in the emerging field of comprehensive multidimensional chromatography.

    Nanoflow LC–Q-TOF MS for De Novo Peptide Sequencing in Microbial Proteomics



    The authors describe how they have implemented a nanoflow liquid chromatography approach to combine high throughput with the sensitivity necessary to allow protein identification from 2D-PAGE separations.

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