Another precaution I recommend is not to shut off the LC system for more than an hour or so if it or the column contains buffer.

What causes split or fronting peaks?

Do sticky check valves have you discouraged?

Are you stuck on which step to take next?

What do you do when the method can't be reproduced?

Ion-pair chromatography can be your best friend or your worst enemy.

If the problem source cannot be found or if found, cannot be corrected, an adjustment in flow-rate is justified.

A very efficient way to start an isocratic method development project is to make the first run as a scouting gradient run.

Trade extra resolution for speed.