If the problem source cannot be found or if found, cannot be corrected, an adjustment in flow-rate is justified.

A very efficient way to start an isocratic method development project is to make the first run as a scouting gradient run.

Trade extra resolution for speed.

How to avoid an expensive shot in the dark.

How do I get the most out of my efforts?

Faster isn't always better.

Getting started on the right foot is important for efficient method development.

Different methods require different strategies.

Column temperature plays an important role in controlling peak spacing (selectivity) in reversed-phase liquid chromatography (LC) separations. Temperature has long been known to affect retention time, and more recently, its use in adjusting selectivity has gained popularity (see reference 1 for a review of temperature selectivity). In preparation of a paper for the most recent Pittsburgh Conference, I had an opportunity to reexamine some data that compare temperature selectivity with other variables used to control selectivity in LC separation. This month's instalment of "LC Troubleshooting" examines temperature selectivity and its relationship to pH selectivity.